Recombinant Human Transcription Factor B1, Mitochondrial, His-tagged

• Cat.No.: TFB1M-1785H
• Product Overview: Recombinant humanTFB1M protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: TFB1M belongs to themethyltransferase superfamily. TFB1M is a dimethyltransferase that methylatesmitochondrial 12S rRNA at the conserved stem loop. This protein also is partof the basal mitochondrial transcription complex and is necessary formitochondrial gene expression. TFB1M stimulates transcription independentlyof the methyltransferase activity.
• Concentration: 0.5 mg/ml
• Form: Suppliedas a liquid in 20mM Tris-HCl buffer, pH 8.0, with 20% glycerol, 0.1M NaCl and1mM DTT.
• Purity: > 90% by SDS -PAGE
• Sequence: 28-346 amino acids:MGSSHHHHHH SSGLVPRGSH MQAAKQLSQN FLLDLRLTDK IVRKAGNLTN AYVYEVGPGP GGITRSILNADVAELLVVEK DTRFIPGLQM LSDAAPGKLR IVHGDVLTFK VEKAFSESLK RPWEDDPPNV HIIGNLPFSVSTPLIIKWLE NISCRDGPFV YGRTQMTLTF QKEVAERLAA NTGSKQRSRL SVMAQYLCNV RHIFTIPGQAFVPK
• Molecular Mass: 38.8 kDa.
• Applications: SDS-PAGE
• Storage: Storeat 4 deg C for short term storage. Aliquot and store at -20 deg C or -70 degC for long term storage. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: TFB1M

Gene Information

• Gene Name: TFB1M transcription factor B1,mitochondrial [ Homo sapiens ]
• Synonyms: TFB1M;transcription factor B1, mitochondrial; CGI75; mtTFB;CGI-75; mtTFB1; dimethyladenosine transferase 1, mitochondrial; hTFB1M;h-mtTFB1; mitochondrial 12S rRNA dimethylase 1; mitochondrialdimethyladenosine transferase 1; homolog of yeast mitochondrial transcriptionfactor B; S-adenosylmethionine-6-N", N"-adenosyl(rRNA) dimethyltransferase 1;Dimethyladenosinetransferase 1, mitochondrial; Mitochondrial 12S rRNA dimethylase 1; Mitochondrialtranscription factor B1; h-mtTFB;S-adenosylmethionine-6-N",N"-adenosyl(rRNA) dimethyltransferase 1; EC 2.1.1.- ; OTTHUMP00000017473
• Gene ID: 51106
• mRNA Refseq: NM_057104
• Protein Refseq: NP_016020
• MIM: 607033
• UniProt ID: Q8WVM0
• Chromosome Location: 6q25.1-q25.3
• Pathway: Energy Metabolism;Mitochondrial Gene Expression
• Function: DNA binding; protein binding;methyltransferase activity; rRNA (adenine-N6, N6)-dimethyltransferaseactivity; transferase activity