Recombinant Human TFB1M, His-tagged

• Cat.No.: TFB1M-3619H
• Product Overview: Recombinant human TFB1M protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: TFB1M belongs to the methyltransferase superfamily. TFB1M is a dimethyltransferase that methylates mitochondrial 12S rRNA at the conserved stem loop. This protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial gene expression. TFB1M stimulates transcription independently of the methyltransferase activity.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Molecular Weight: 38.8 kDa (340aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MQAAKQLSQN FLLDLRLTDK IVRKAGNLTN AYVYEVGPGP GGITRSILNA DVAELLVVEK DTRFIPGLQM LSDAAPGKLR IVHGDVLTFK VEKAFSESLK RPWEDDPPNV HIIGNLPFSV STPLIIKWLE NISCRDGPFV YGRTQMTLTF QKEVAERLAA NTGSKQRSRL SVMAQYLCNV RHIFTIPGQA FVPKPEVDVG VVHFTPLIQP KIEQPFKLVE KVVQNVFQFR RKYCHRGLRM LFPEAQRLES TGRLLELADI DPTLRPRQLS ISHFKSLCDV YRKMCDEDPQ LFAYNFREEL KRRKSKNEEK EEDDAENYRL
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: TFB1M transcription factor B1, mitochondrial [ Homo sapiens ]
• Official Symbol: TFB1M
• Synonyms: TFB1M; CGI75; mtTFB; CGI-75; mtTFB1; transcription factor B1, mitochondrial; dimethyladenosinetransferase 1, mitochondrial; hTFB1M; h-mtTFB1; OTTHUMP00000017473; mitochondrial 12S rRNAdimethylase 1; mitochondrial dimethyladenosinetransferase 1; homolog of yeast mitochondrial transcription factor B; S-adenosylmethionine-6-N", N"-adenosyl(rRNA) dimethyltransferase 1; EC 2.1.1.-; Mitochondrial transcription factor B1; h-mtTFB
• Gene ID: 51106
• mRNA Refseq: NM_016020
• Protein Refseq: NP_057104
• MIM: 607033
• UniProt ID: Q8WVM0
• Chromosome Location: 6q25.1-q25.3
• Function: DNA binding; methyltransferase activity; protein binding; rRNA (adenine-N6,N6-)-dimethyltransferase activity; transferase activity