Recombinant Human TPX2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TPX2-1921H
• Product Overview: TPX2 MS Standard C13 and N15-labeled recombinant protein (NP_036244) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 85.7 kDa
• AA Sequence: MSQVKSSYSYDAPSDFINFSSLDDEGDTQNIDSWFEEKANLENKLLGKNGTGGLFQGKTPLRKANLQQAIVTPLKPVDNTYYKEAEKENLVEQSIPSNACSSLEVEAAISRKTPAQPQRRSLRLSAQKDLEQKEKHHVKMKAKRCATPVIIDEILPSKKMKVSNNKKKPEEEGSAHQDTAEKNASSPEKAKGRHTVPCMPPAKQKFLKSTEEQELEKSMKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKDDINLLPSKSSVTKICRDPQTPVLQTKHRARAVTCKSTAELEAEELEKLQQYKFKARELDPRILEGGPILPKKPPVKPPTEPIGFDLEIEKRIQERESKKKTEDEHFEFHSRPCPTKILEDVVGVPEKKVLPITVPKSPAFALKNRIRMPTKEDEEEDEPVVIKAQPVPHYGVPFKPQIPEARTVEICPFSFDSRDKERQLQKEKKIKELQKGEVPKFKALPLPHFDTINLPEKKVKNVTQIEPFCLETDRRGALKAQTWKHQLEEELRQQKEAACFKARPNTVISQEPFVPKKEKKSVAEGLSGSLVQEPFQLATEKRAKERQELEKRMAEVEAQKAQQLEEARLQEEEQKKEELARLRRELVHKANPIRKYQGLEIKSSDQPLTVPVSPKFSTRFHCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TPX2 TPX2 microtubule nucleation factor [ Homo sapiens (human) ]
• Official Symbol: TPX2
• Synonyms: TPX2; TPX2, microtubule-associated, homolog (Xenopus laevis); C20orf1, C20orf2, chromosome 20 open reading frame 1; targeting protein for Xklp2; DIL 2; p100; protein fls353; differentially expressed in lung cells; TPX2, microtubule-associated protein homolog; preferentially expressed in colorectal cancer; hepatocellular carcinoma-associated antigen 519; restricted expression proliferation associated protein 100; restricted expression proliferation-associated protein 100; differentially expressed in cancerous and non-cancerous lung cells 2; DIL2; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
• Gene ID: 22974
• mRNA Refseq: NM_012112
• Protein Refseq: NP_036244
• MIM: 605917
• UniProt ID: Q9ULW0