Recombinant Human TPMT Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TPMT-5121H
• Product Overview: TPMT MS Standard C13 and N15-labeled recombinant protein (NP_000358) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 28.2 kDa
• AA Sequence: MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRCLEKVDAFEERHKSWGIDCLFEKLYLLTEKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TPMT thiopurine S-methyltransferase [ Homo sapiens (human) ]
• Official Symbol: TPMT
• Synonyms: TPMT; thiopurine S-methyltransferase; S-adenosyl-L-methionine:thiopurine S-methyltransferase
• Gene ID: 7172
• mRNA Refseq: NM_000367
• Protein Refseq: NP_000358
• MIM: 187680
• UniProt ID: P51580