Recombinant Human Tousled-Like Kinase 1, GST-tagged

• Cat.No.: TLK1-1112H
• Product Overview: Recombinant full-length human TLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: TLK1 or tousled-like kinase 1 is a nuclear serine/threonine kinase that is potentially involved in the regulation of chromatin assembly. TLK1 is first described in Arabidopsis. TLK1 is a novel target of the DNA damage checkpoint that is rapidly inactivated upon exposure to ionizing radiation (IR) and the inactivation is directly mediated by the S-phase DNA damage checkpoint. The suppression of TLK1 activity after double-strand breaks in the DNA, replication blockade, or low doses of ultraviolet irradiation requires ATM, NBS and CHK1.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 135 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
• Specific Activity: 6.2 nmol/min/mg
• Purity: > 90 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: TLK1 tousled-like kinase 1 [ Homo sapiens ]
• Official Symbol: TLK1
• Synonyms: TLK1; tousled-like kinase 1; KIAA0137; PKU-beta; Serine/threonine-protein kinase tousled-like 1; EC=2.7.11.1; Tousled-like kinase 1; serine threonine protein kinase; SNARE protein kinase SNAK; EC 2.7.11; EC 2.7.11.1; PKU-BETA; OTTHUMP00000205035; OTTHUMP00000205036; OTTHUMP00000205037; OTTHUMP00000205038; OTTHUMP00000205039; OTTHUMP00000225037; OTTHUMP00000163076; OTTHUMP00000163077
• Gene ID: 9874
• mRNA Refseq: NM_012290
• Protein Refseq: NP_036422
• MIM: 608438
• UniProt ID: Q9UKI8
• Chromosome Location: 2q31.1
• Function: ATP binding; protein serine/ threonine kinase activity; nucleotide binding