Recombinant Human TNRC6A protein, His & T7-tagged

• Cat.No.: TNRC6A-8080H
• Product Overview: Recombinant Human TNRC6A aa. (Thr1670~Met1962 (Accession # Q8NDV7)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Thr1670~Met1962
• Description: This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.9kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.4.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: TNRC6A trinucleotide repeat containing 6A [ Homo sapiens (human) ]
• Official Symbol: TNRC6A
• Synonyms: GW1; GW182; TNRC6; CAGH26; CAG repeat protein 26; CTD-2540M10.1; EDIE; EMSY interactor protein; GW182 autoantigen; glycine-tryptophan protein of 182 kDa; trinucleotide repeat-containing gene 6A protein
• Gene ID: 27327
• mRNA Refseq: NM_001330520.2
• Protein Refseq: NP_001317449.1
• UniProt ID: Q8NDV7