Recombinant Human TNFSF9 Protein, His-tagged

• Cat.No.: TNFSF9-70H
• Product Overview: Recombinant Human 4-1BB Ligand is produced by our E.coli expression system and the target gene encoding Arg71-Glu254 is expressed with a 6His tag at the C-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 71-254 a.a.
• Description: Tumor necrosis factor ligand superfamily member 9, also known as 4-1BBL, is a member of the the tumor necrosis factor family. It is a 254 amino acids cytokine that is expressed in brain, placenta, lung, skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells, and is thought to be involved in T cell-tumor cell interaction.
• Form: Lyophilized from a 0.2 um filtered solution of 20mM PB，150mM NaCl, pH7.4.
• Molecular Mass: Predicted Molecular Weight: 20.6KDa
  Apparent Molecular Weight: 16-20kDa, reducing conditions.
• AA Sequence: Arg71-Glu254
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: >95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: TNFSF9 TNF superfamily member 9 [ Homo sapiens (human) ]
• Official Symbol: TNFSF9
• Synonyms: Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL; CD137L; TNLG5A
• Gene ID: 8744
• mRNA Refseq: NM_003811.4
• Protein Refseq: NP_003802.1
• MIM: 606182
• UniProt ID: P41273