Active Recombinant Mouse Tnfsf9 Protein (Arg104-Glu309), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Tnfsf9-88M
• Product Overview: Recombinant Mouse Tnfsf9 Protein (Arg104-Glu309) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Arg104-Glu309
• Description: 4-1BB ligand (4-1BBL) is a type II transmembrane protein that is part of the tumor necrosis factor (TNF) ligand family. As an inducible co-stimulatory molecule, it presents on several antigen presenting cell (APC) types, including B cells, macrophages and DCs. The interactions between 4-1BB and 4-1BBL trigger pleiotropic effects on the immune response including antigen presenting process, proliferation of CD4 and CD8 positive T-cells, as well as cytokine secretion from T-cells through NFκB, c-Jun, and p38 downstream signal pathways activation. Therefore, 4-1BB and 4-1BBL are recently used for the immunotherapy of cancer.
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce IL-2 secretion in mouse T cells in the presence of the anti-CD3 antibody. The ED50 for this effect is < 0.05 μg/mL.
• Molecular Mass: The protein has a calculated MW of 23.9 kDa.
  The protein migrates as 24 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Tnfsf9 tumor necrosis factor (ligand) superfamily, member 9 [ Mus musculus (house mouse) ]
• Official Symbol: Tnfsf9
• Synonyms: TNFSF9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817
• Gene ID: 21950
• mRNA Refseq: NM_009404
• Protein Refseq: NP_033430
• UniProt ID: P41274