Recombinant Human TNFSF15 Trimer protein, N-His-Flag-tagged

• Cat.No.: TNFSF15-150H
• Product Overview: Recombinant Human TNFSF15 Trimer protein(O95150-1)(Asp91-Leu251), fused with N-terminal Flag tag and His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N-Flag-His
• Protein length: Asp91-Leu251
• Form: Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TNFSF15 Trimer, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA. The affinity constant of 3.06 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per ug by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNFSF15 tumor necrosis factor (ligand) superfamily, member 15 [ Homo sapiens ]
• Official Symbol: TNFSF15
• Synonyms: TNFSF15; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand superfamily member 15; MGC129934; MGC129935; TL1; TL1A; TNF ligand related molecule 1; TNF superfamily ligand TL1A; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor 192A; VEGI; VEGI192A; TNF ligand-related molecule 1; vascular endothelial growth inhibitor-192A
• Gene ID: 9966
• mRNA Refseq: NM_001204344
• Protein Refseq: NP_001191273
• MIM: 604052
• UniProt ID: O95150