Recombinant Human TNFRSF10A protein, His-Avi-tagged, Biotinylated

• Cat.No.: TNFRSF10A-212HB
• Product Overview: Biotinylated Recombinant Human TNFRSF10A protein(O00220)(Pro34-Asn239), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Pro34-Asn239
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.31ug/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TNFRSF10A tumor necrosis factor receptor superfamily, member 10a [ Homo sapiens ]
• Official Symbol: TNFRSF10A
• Synonyms: TNFRSF10A; tumor necrosis factor receptor superfamily, member 10a; tumor necrosis factor receptor superfamily member 10A; Apo2; CD261; DR4; TRAILR 1; TRAIL-R1; TRAIL receptor 1; death receptor 4; cytotoxic TRAIL receptor; TNF-related apoptosis-inducing ligand receptor 1; tumor necrosis factor receptor superfamily member 10a variant 2; APO2; TRAILR1; TRAILR-1; MGC9365
• Gene ID: 8797
• mRNA Refseq: NM_003844
• Protein Refseq: NP_003835
• MIM: 603611
• UniProt ID: O00220