Recombinant Human TNF Protein, His-tagged

• Cat.No.: TNF-001H
• Product Overview: Recombinant Human TNF Protein (77-233 aa (partial, without signal peptide)) with 6xHis tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 77-233
• Description: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
• Molecular Mass: 18.4 kDa
• Purity: >90% confirmed by 10% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-TNFa (WB).
• Applications: ELISA, Western Blot, Antibody Production, Protein array
• Quality Control Test: 15% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-TNFa (WB) and peptide fingerprinting by MALDI-TOF mass spectrometry.
• Storage: Prior to reconstitution, store the lyophilized protein at 2−8 centigrade. After reconstitution, store in working aliquots at −80 centigrade.
• Expiry: All components are stable for 1 year of receipt, if stored properly.
• Storage Buffer: 20mM Sodium Phosphate, 0.5M NaCl, pH 7.4. No preservatives added.
• Shipping: Lyophilized at Ambient temperature

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens (human) ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• UniProt ID: P01375