Recombinant Rat Tnf, His-tagged

• Cat.No.: Tnf-108R
• Product Overview: Recombinant rat Tnf, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Description: Tnf also known as Tumor necrosis factor. The Protein acts as a cytokine binds TNF receptors plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response.
• Form: Liquid. In Phosphate Buffer Saline (pH7.4) containing 10% glycerol, 1mM DTT
• Molecular Mass: 19.9kDa (181aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFKGQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIAL
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Tnf tumor necrosis factor [ Rattus norvegicus ]
• Official Symbol: Tnf
• Synonyms: TNF; tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; Tnfa; RATTNF; TNF-alpha; MGC124630
• Gene ID: 24835
• mRNA Refseq: NM_012675
• Protein Refseq: NP_036807
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem
• Function: cytokine activity; cytokine activity; identical protein binding; identical protein binding; protease binding; protease binding; transcription regulatory region DNA binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor binding