Recombinant Human TLR3 protein, His-tagged, Biotinylated

• Cat.No.: TLR3-1726H
• Product Overview: Biotinylated Recombinant Human TLR3 protein(Q6PCD4)(Ser23-Glu703), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: C-His
• Tag: Non
• Protein Length: Ser23-Glu703
• Tag: C-His
• Conjugation/Label: Biotin
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human TLR3, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 78.7 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TLR3 toll-like receptor 3 [ Homo sapiens ]
• Official Symbol: TLR3
• Synonyms: TLR3; toll-like receptor 3; CD283; IIAE2
• Gene ID: 7098
• mRNA Refseq: NM_003265
• Protein Refseq: NP_003256
• MIM: 603029
• UniProt ID: O15455