Recombinant Human TLR3 protein, His-tagged

• Cat.No.: TLR3-151H
• Product Overview: Recombinant Human TLR3 protein(Q6PCD4)(Ser23-Glu703), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ser23-Glu703
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TLR3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 78.7 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TLR3 toll-like receptor 3 [ Homo sapiens ]
• Official Symbol: TLR3
• Synonyms: TLR3; toll-like receptor 3; CD283; IIAE2
• Gene ID: 7098
• mRNA Refseq: NM_003265
• Protein Refseq: NP_003256
• MIM: 603029
• UniProt ID: O15455