Recombinant Human Toll-like Receptor 3

• Cat.No.: TLR3-975H
• Product Overview: Recombinant human TLR-3 produced in293 cellsis 77.0 kDa glycoprotein containing 681 residues which comprise the TLR-3 extracellular domain.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: TLR-3 is a single-pass type I receptor that binds to and signals the presence of microbial pathogens and double stranded RNA (dsRNA) viruses. Signaling through TLR-3 can promote the NF-κB pathway to initiate innate and adaptive immune responses to bacterial and viral infections, as well as the p53 pathway to trigger apoptosis in cells infected with dsRNA viruses. TLR-3 belongs to a family of structurally-related toll-like receptors (TLRs) containing an N-terminal domain rich in leucine repeats and a C-terminal intracellular Toll/interleukin (IL)-1 (TIL) domain. TLR-3 is expressed primarily in dendritic cells of the placenta and pancreas where it can reside on both sides of the plasma membrane and in the endosomal compartment of the cells.
• Purity: 95 % (SDS-PAGE, HPLC).
• Endotoxin level: < 0.1 ng per μg of TLR-3.
• Stabilizer: None.
• Formulation: Lyophilized.
• Biologically Activity: Determined by its ability to inhibit IL-8 secretion induced by Poly I:C in the TLR3 receptor-expressing HEK293 cells (HEK293 TLR3r). The ED50for this effect is ≤ 30 μg/ml in the presence of 100 μg/ml of Poly I:C.
• Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted TLR-3 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: TLR3 toll-like receptor 3 [ Homo sapiens ]
• Synonyms: TLR3; toll-like receptor 3; CD283
• Gene ID: 7098
• mRNA Refseq: NM_003265
• Protein Refseq: NP_003256
• MIM: 603029
• UniProt ID: O15455
• Chromosome Location: 4q35
• pathway: Toll-like receptor signaling pathway
• Function: double-stranded RNA binding; protein binding; transmembrane receptor activity