Recombinant Human TJP2 293 Cell Lysate

• Cat.No.: TJP2-1055HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: TJP2 tight junction protein 2 (zona occludens 2) [ Homo sapiens ]
• Official Symbol: TJP2
• Synonyms: TJP2; tight junction protein 2 (zona occludens 2); deafness, autosomal dominant 51 , DFNA51; tight junction protein ZO-2; Friedreich ataxia region gene X104 (tight junction protein ZO 2); X104; ZO 2; ZO2; zonula occludens protein 2; Friedreich ataxia region gene X104 (tight junction protein ZO-2); DFNA51; DUP9q21.11; C9DUPq21.11; MGC26306
• Gene ID: 9414
• mRNA Refseq: NM_001170414
• Protein Refseq: NP_001163885
• MIM: 607709
• UniProt ID: Q9UDY2
• Chromosome Location: 9q13-q21
• Pathway: Apoptosis, organism-specific biosystem; Apoptotic cleavage of cell adhesionproteins, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by Hippo, organism-specific biosystem; Tight junction, organism-specific biosystem