Recombinant Human Thymidine Kinase 2, Mitochondrial, His-tagged

• Cat.No.: TK2-1350H
• Product Overview: Recombinant human TK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 34-265aa
• Description: TK2 belongs to the DCK/DGK family. Thymidine kinase is an enzyme, a phosphotransferase (a kinase): 2"-deoxythymidine kinase, ATP-thymidine 5"-phosphotransferase, It can be found in most living cells. It is present in two forms in mammalian cells, TK1 and TK2. Certain viruses also have genetic information for expression of viral thymidine kinases. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA.
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 30% glycerol, 2mM DTT, 200mM NaCl.
• Molecular Mass: 30.2 kDa (257aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMVQRRA WPPDKEQEKE KKSVICVEGN IASGKTTCLE FFSNATDVEV LTEPVSKWRN VRGHNPLGLM YHDASRWGLT LQTYVQLTML DRHTRPQVSS VRLMERSIHS ARYIFVENLY RSGKMPEVDY VVLSEWFDWI LRNMDVSVDL IVYLRTNPET CYQRLKKRCR EEEKVIPLEY LEAIHHLHEE WLIKGSLFPM AAPVLVIEAD HHMERMLELF EQNRDRILTP ENRKHCP
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TK2 thymidine kinase 2, mitochondrial [ Homo sapiens ]
• Official Symbol: TK2
• Synonyms: TK2; thymidine kinase 2, mitochondrial; mt-TK; MTTK; MTDPS2
• Gene ID: 7084
• mRNA Refseq: NM_001172643
• Protein Refseq: NP_001166114
• MIM: 188250
• UniProt ID: O00142
• Chromosome Location: 16q22-q23.1
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Drug metabolism - other enzymes, organism-specific biosystem; Drug metabolism - other enzymes, conserved biosystem; E2F mediated regulation of DNA replication, organism-specific biosystem; G1/S Transition, organism-specific biosystem; G1/S-Specific Transcription, organism-specific biosystem
• Function: ATP binding; kinase activity; nucleotide binding; phosphotransferase activity, alcohol group as acceptor; thymidine kinase activity; transferase activity