Recombinant Human Thioredoxin2

• Cat.No.: TXN2-133H
• Product Overview: Recombinant Thioredoxin2 was expressed in E.coli and purified by using conventional chromatography techniques, 11 kDa (108 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The Thioredoxin2 may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis.
• Sequences of amino acids: MTTFNIQDGP DFQDRVVNSE TPVVVDFHAQ WCGPCKILGP RLEKMVAKQH GKVVMAKVDI DDHTDLAIEY EVSAVPTVLA MKNGDVVDKF VGIKDEDQLE AFLKKLIG
• Formulation: Liquid. In PBS (pH 7.4)
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Biological activity: Specific activity is 3-4 A650/min/mg and was obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
• Activity Assay: 1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant thioredoxin2 into reaction cocktail and mix immediately. 3. Incubate at 25C for 20 minutes. 4. Record the increase in A650nm for 15 minutes.
• Storagee: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• GeneIDD: 25828

Gene Information

• Synonyms: MT-TRX; MTRX; Mt-Trx; TRX2; Thioredoxin-2; mitochondrial thioredoxin ; thioredoxin 2; TXN2
• mRNA Refseq: NM_012473
• Protein Refseq: NP_036605
• MIM: 609063
• UniProt ID: Q99757
• Chromosome Location: 22q13.1
• Function: electron carrier activity; protein disulfide oxidoreductase activity