Recombinant Human TFF2,His-tagged

• Cat.No.: TFF2-1045H
• Product Overview: Total 116 AA. MW: 13,2 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Specification

• Cat. No.: TFF2-1045H
• Description: Trefoil factor 2 is a protein that in humans is encoded by the TFF2 gene. It belongs to the TFF protein family that is characterized by a clover leaf?Clike disulphide structure named the TFF domain, which is created by 6 cysteines forming three intramolecular bonds. TFF2 contains two trefoil domains and has a very compact structure which may account for its extremely high resistance against the harsh environment in the gastrointestinal tract.
• Source: E. coli
• Species: Human
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 20mM NaCl, pH 7.5.
• Research topic: Energy metabolism and body weight regulation, Immune Response, Infection and Inflammation, Oncology, Sepsis
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: ELISA; Western blotting
• Quality Control Test: BCA to determine quantity of the protein;SDS PAGE to determine purity of the protein;LAL to determine quantity of endotoxin.
• Sequences of amino acids: MKHHHHHHAS EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
• Protein length: 24-129 a.a.

Gene Information

• Gene Name: TFF2 trefoil factor 2 [ Homo sapiens ]
• Official Symbol: TFF2
• Synonyms: TFF2; trefoil factor 2; SML1; spasmolytic protein 1; Spasmolytic polypeptide; SP; trefoil factor 2, SML1, human spasmolytic polypeptide (SP); spasmolysin; Spasmolysin
• Gene ID: 7032
• mRNA Refseq: NM_005423
• Protein Refseq: NP_005414
• MIM: 182590
• Uniprot ID: Q03403
• Chromosome Location: 21q22.3