Recombinant human TAF15, His-tagged

• Cat.No.: TAF15-2466H
• Product Overview: Recombinant human TAF15 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: TAF15 is a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT.
• Molecular Mass: 30kDa (282aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSSYHSQRE NYSHHTQDDR RDVSRYGEDN RGYGGSQGGG RGRGGYDKDG RGPMTGSSGG DRGGFKNFGGHRDYGPRTDA DSESDNSDNN TIFVQGLGEG VSTDQVGEFF KQIGIIKTNK KTGKPMINLY TDKDTGKPKG EATVSFDDPP SAKAAIDWFD GKEFHGNIIK VSFATRRPEF MRGGGSGGGR RGRGGYRGRG GFQGRGGDPK SGDWVCPNPS CGNMNFARRN SCNQCNEPRP EDSRPSGGDFRGRGYGGERG YR
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Protein length: 148-406 aa

Gene Information

• Gene Name: TAF15 TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa [ Homo sapiens ]
• Official Symbol: TAF15
• Synonyms: TAF15; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa; TAF2N, TATA box binding protein (TBP) associated factor, RNA polymerase II, N, 68kD (RNA binding protein 56); TATA-binding protein-associated factor 2N; hTAFII68; Npl3; RBP56; TAF(II)68; RBP56/CSMF fusion; TBP-associated factor 15; 68 kDa TATA-binding protein-associated factor; TATA box-binding protein-associated factor 2N (RNA-binding protein 56); TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56); TAF2N; TAFII68
• Gene ID: 8148
• mRNA Refseq: NM_003487
• Protein Refseq: NP_003478
• MIM: 601574
• UniProt ID: Q92804
• Chromosome Location: 17q11.1-q11.2
• Pathway: Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem
• Function: DNA binding; RNA binding; metal ion binding; nucleotide binding; protein binding; zinc ion binding