Recombinant Human Synaptosomal-Associated Protein, 25kDa

• Cat.No.: SNAP25-6928H
• Product Overview: Recombinant SNAP25 protein was expressed in E. coli and purified by using conventional chromatography techniques. MW: 23 kDa.

Specification

• Description: SNAP25 is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters and this protein exists as two alternative isoforms, SNAP25A and SNAP25B which differ by 9 amino acids in central portion of these proteins.
• Source: E. coli
• Species: Human
• Tag: Non
• Form: Liquid in 25mM Tris pH 7.5, 1mM DTT, 2mM EDTA, 10% glycerol
• Molecular Mass: 23 kDa (206 aa), confirmed by MALDI-TOF.
• Protein length: 1-206aa
• AA Sequence: MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SNAP25 synaptosomal-associated protein, 25kDa [ Homo sapiens ]
• Official Symbol: SNAP25
• Synonyms: SNAP25; synaptosomal-associated protein, 25kDa; SNAP, synaptosomal associated protein, 25kD; synaptosomal-associated protein 25; bA416N4.2; dJ1068F16.2; resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC9; SNAP 25; SUP; super protein; SNAP; RIC-4; SNAP-25; FLJ23079
• Gene ID: 6616
• mRNA Refseq: NM_003081
• Protein Refseq: NP_003072
• MIM: 600322
• UniProt ID: P60880
• Chromosome Location: 20p12-p11.2
• Pathway: Acetylcholine Neurotransmitter Release Cycle, organism-specific biosystem; BoNT Light Chain Types A, C1, E cleave SNAP-25, organism-specific biosystem; Botulinum neurotoxicity, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Dopamine Neurotransmitter Release Cycle, organism-specific biosystem; Effects of Botulinum toxin, organism-specific biosystem
• Function: SNARE binding; myosin binding; protein N-terminus binding; protein binding; protein domain specific binding; syntaxin-1 binding; voltage-gated potassium channel activity