Recombinant Human Suppressor Of fused Homolog (Drosophila), His-tagged

• Cat.No.: SUFU-6871H
• Product Overview: Recombinant human SUFU protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 56.1 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-484aa
• Description: SUFU (Suppressor of fused homolog) belongs to the SUFU family. This protein is negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. SUFU has been shown to interact with GLI1, GLI3 and PEX26.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 50mM NaCl.
• Molecular Mass: 56.1 kDa (504aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MAELRPSGAP GPTAPPAPGP TAPPAFASLF PPGLHAIYGE CRRLYPDQPN PLQVTAIVKY WLGGPDPLDY VSMYRNVGSP SANIPEHWHY ISFGLSDLYG DNRVHEFTGT DGPSGFGFEL TFRLKRETGE SAPPTWPAEL MQGLARYVFQ SENTFCSGDH VSWHSPLDNS ESRIQHMLLT EDPQMQPVQT PFGVVTFLQI VGVCTEELHS AQQWNGQGIL ELLRTVPIAG GPWLITDMRR GETIFEIDPH LQERVDKGIE TDGSNLSGVS AKCAWDDLSR PPEDDEDSRS ICIGTQPRRL SGKDTEQIRE TLRRGLEINS KPVLPPINPQ RQNGLAHDRA PSRKDSLESD SSTAIIPHEL IRTRQLESVH LKFNQESGAL IPLCLRGRLL HGRHFTYKSI TGDMAITFVS TGVEGAFATE EHPYAAHGPW LQILLTEEFV EKMLEDLEDL TSPEEFKLPK EYSWPEKKLK VSILPDVVFD SPLH
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SUFU suppressor of fused homolog (Drosophila) [ Homo sapiens ]
• Official Symbol: SUFU
• Synonyms: SUFU; suppressor of fused homolog (Drosophila); suppressor of fused homolog; PRO1280; SUFUH; SUFUXL
• Gene ID: 51684
• mRNA Refseq: NM_001178133
• Protein Refseq: NP_001171604
• MIM: 607035
• UniProt ID: Q9UMX1
• Chromosome Location: 10q24.32
• Pathway: Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Hedgehog Signaling Pathway, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem; Hedgehog signaling pathway, organism-specific biosystem; Hedgehog signaling pathway, conserved biosystem; Pathways in cancer, organism-specific biosystem
• Function: beta-catenin binding; protein binding; protein kinase binding; signal transducer activity; transcription corepressor activity; transcription factor binding