Recombinant Mouse Sufu Protein, Myc/DDK-tagged

Cat.No. : Sufu-6218M
Product Overview : Purified recombinant protein of mouse full-length SUFU negative regulator of hedgehog signaling (Sufu), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
  • Specification
  • Gene Information
  • Related Products
  • Download
Species : Mouse
Source : HEK293
Tag : DDK&Myc
Description : Negative regulator in the hedgehog/smoothened signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full-length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for normal embryonic development. Required for the proper formation of hair follicles and the control of epidermal differentiation.
Molecular Mass : 54 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name Sufu SUFU negative regulator of hedgehog signaling [ Mus musculus (house mouse) ]
Official Symbol Sufu
Synonyms SUFU; suppressor of fused homolog (Drosophila); suppressor of fused homolog; Su(fu); 2810026F04Rik
Gene ID 24069
mRNA Refseq NM_001025391
Protein Refseq NP_001020562
UniProt ID Q9Z0P7

Not For Human Consumption!

Inquiry

  • Reviews
  • Q&As

Customer Reviews (0)

Write a review

Q&As (0)

Ask a question

Ask a Question for All Sufu Products

Required fields are marked with *

My Review for All Sufu Products

Required fields are marked with *

0

Inquiry Basket

cartIcon