Recombinant Human Stratifin, GST-tagged

• Cat.No.: SFN-1421H
• Product Overview: Recombinant human full-length 14-3-3sigma was expressed inE. colicells using a N-terminal GST tag. MW=51kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: 14-3-3σ or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3σ is mediated by a p53 responsive element. Exogenous introduction of 14-3-3σ into cycling cells results in a G2 cell cycle arrest. Knockout of 14-3-3σ in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3σ -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3σ -deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3σ plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death.
• Sequence: Full-length.
• Applications: Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: SFN stratifin [ Homo sapiens ]
• Synonyms: SFN; stratifin; YWHAS; 14-3-3 sigma; 14-3-3 protein sigma; Stratifin; Epithelial cell marker protein 1
• Gene ID: 2810
• mRNA Refseq: NM_006142
• Protein Refseq: NP_006133
• UniProt ID: P31947
• Chromosome Location: 1p36.11
• MIM: 601290
• Pathway: Cell Cycle; p53 signaling pathway
• Function: protein kinase C inhibitor activity; protein domain specific binding