Recombinant Human SFN Protein, His-tagged

• Cat.No.: SFN-149H
• Product Overview: Recombinant Human SFN fused with His tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: 14-3-3 sigma or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3 sigma is mediated by a p53 responsive element. Exogenous introduction of 14-3-3 sigma into cycling cells results in a G2 cell cycle arrest. Knockout of 14-3-3 sigma in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3sigma -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3sigma-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3 sigma plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death.
• Form: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
• Molecular Mass: 30 kDa
• Purity: > 95%
• Applications: Western Blot
• Stability: 1 year at -70 centigrade from date of shipment.
• Storage: Store product at –70centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.2 ug/ml

Gene Information

• Gene Name: SFN stratifin [ Homo sapiens ]
• Official Symbol: SFN
• Synonyms: SFN; stratifin; 14-3-3 protein sigma; 14 3 3 sigma; YWHAS; 14-3-3 sigma; epithelial cell marker protein 1
• Gene ID: 2810
• mRNA Refseq: NM_006142
• Protein Refseq: NP_006133
• MIM: 601290
• UniProt ID: P31947