Recombinant Human SRF protein, His & T7-tagged

• Cat.No.: SRF-2719H
• Product Overview: Recombinant Human SRF aa. (Thr231~Gln474 (Accession # P11831)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Thr231~Gln474
• Description: This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.5kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.9.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: SRF serum response factor [ Homo sapiens (human) ]
• Official Symbol: SRF
• Synonyms: MCM1; c-fos serum response element-binding transcription factor; minichromosome maintenance 1 homolog; serum response factor
• Gene ID: 6722
• mRNA Refseq: NM_001292001.1
• Protein Refseq: NP_001278930.1
• UniProt ID: P11831