Recombinant Human src tyrosine kinase Protein, His tagged

• Cat.No.: CSK-009H
• Product Overview: Recombinant human CSK protein (P41240) (Met 173-Leu 450) with a His tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 173-450aa
• Description: Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
• Tag: C-His
• Molecular Mass: 32.9 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: CSK c-src tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: CSK
• Synonyms: CSK; c-src tyrosine kinase; tyrosine-protein kinase CSK; C-Src kinase; protein-tyrosine kinase CYL; MGC117393
• Gene ID: 1445
• mRNA Refseq: NM_001127190
• Protein Refseq: NP_001120662
• MIM: 124095
• UniProt ID: P41240