Recombinant Human SPP1 Protein, MBP-tagged

• Cat.No.: SPP1-001H
• Product Overview: Recombinant Human SPP1 Protein (1-300 aa (full-length)) with MBP tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: MBP
• Molecular Mass: 78.5 kDa
• Protein length: 1-300
• Purity: >80% confirmed by 10% SDS-PAGE stained with Coomassie Blue (CB).
• Applications: ELISA, Western Blot, Antibody Production, Protein array
• Quality Control Test: 10% SDS-PAGE stained with Coomassie Blue (CB) and peptide fingerprinting by MALDI-TOF mass spectrometry.
• Storage: Store working aliquots at 4 centigrade for up to one week. For extended storage, conserve at −80 centigrade.
• Expiry: 12 months from the date of purchase, if it is properly stored.
• Storage Buffer: 20mM Tris-HCl, 0.2M NaCl, 1mM EDTA, 10mM Maltose, pH 7.4. No preservatives added.
• Shipping: Dry Ice

Gene Information

• Gene Name: SPP1 secreted phosphoprotein 1 [ Homo sapiens (human) ]
• Official Symbol: SPP1
• Synonyms: SPP1; secreted phosphoprotein 1; OPN; BNSP; BSPI; ETA-1; osteopontin; PP1/CALPHA1 fusion; early T-lymphocyte activation 1; nephropontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; urinary stone protein; uropontin
• Gene ID: 6696
• mRNA Refseq: NM_000582
• Protein Refseq: NP_000573
• MIM: 166490
• UniProt ID: P10451