Recombinant Rat Spp1 protein, His-tagged

• Cat.No.: Spp1-571R

Specification

• Product overview: Recombinant Rat Spp1 (P08721) aa. (Leu17~Asn317) fused with His tag was produced in HEK293F cells.
• Description: The sialoprotein that binds osteosarcoma cells; may play a role in osteoclast cell function.
• Source: HEK293F
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.9kDa; Accurate Molecular Mass: 60kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Leu17~Asn317
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: Isoelectric point is 4.3.
• Applications: Cell culture; Activity Assays; In vivo assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Spp1 secreted phosphoprotein 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Spp1
• Synonyms: Spp1; secreted phosphoprotein 1; OSP; osteopontin; SPP-1; Sialoprotein (osteopontin); bone sialoprotein 1; osteopontin-like
• Gene ID: 25353
• mRNA Refseq: NM_012881.2
• Protein Refseq: NP_037013.2
• UniProt ID: P08721