Recombinant Human SPINK1 Protein, N-Met,N-His-tagged

• Cat.No.: SPINK1-15H
• Product Overview: Recombinant Human SPINK1 Protein, fused to His and Met-tag at N-terminus, was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.
• Source: E. coli
• Species: Human
• Tag: N-Met,N-His
• Protein length: Asp24-Cys79
• Form: Supplied as a 0.2 μm filtered solution in MES, NaCl, CaCl2, DTT, Glycerol and Brij-35.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is <10 nM, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 7 kDa
  SDS-PAGE: 8-11 kDa, reducing conditions
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: SPINK1 serine peptidase inhibitor Kazal type 1 [ Homo sapiens (human) ]
• Official Symbol: SPINK1
• Synonyms: pancreatic secretory trypsin inhibitor; PCTT; PCTTSpink3; PSTI; PSTISerine protease inhibitor Kazal-type 1; serine peptidase inhibitor, Kazal type 1; serine protease inhibitor, Kazal type 1; SPINK1; Spink3; TATI; TATITumor-associated trypsin inhibitor; TCP; TATI
• Gene ID: 6690
• mRNA Refseq: NM_003122.5
• Protein Refseq: NP_003113.2
• MIM: 167790
• UniProt ID: P00995