Recombinant Human SPAST protein, His-tagged

• Cat.No.: SPAST-2537H
• Product Overview: Recombinant Human SPAST protein(79-278 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 79-278 aa
• AA Sequence: CQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRK
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: SPAST spastin [ Homo sapiens ]
• Official Symbol: SPAST
• Synonyms: SPAST; spastin; spastic paraplegia 4 (autosomal dominant; spastin) , SPG4; ADPSP; FSP2; KIAA1083; spastic paraplegia 4 protein; spastic paraplegia 4 (autosomal dominant; spastin); SPG4
• Gene ID: 6683
• mRNA Refseq: NM_014946
• Protein Refseq: NP_055761
• MIM: 604277
• UniProt ID: Q9UBP0