Recombinant Human SNCA protein, His-tagged

• Cat.No.: SNCA-166H
• Product Overview: Recombinant Human TACSTD2 protein (Met1-Ala140) was fused to His-tag at the C-terminus and expressed in E.coli.

Specification

• Description: Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.
• Source: E.coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met1
• Form: Lyophilized from 0.22 μm filtered solution in 100 mM NaAC, pH7.0, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 16.3 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: Met1-Ala140
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SNCA
• Official Symbol: SNCA
• Synonyms: PD1; NACP; PARK1; PARK4
• Gene ID: 6622
• mRNA Refseq: NM_000345.4
• Protein Refseq: NP_000336.1
• MIM: 163890
• UniProt ID: P37840