Recombinant Human SNCA 293 Cell Lysate

• Cat.No.: SNCA-1634HCL

Specification

• Description: Antigen standard for synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: SNCA synuclein, alpha (non A4 component of amyloid precursor) [ Homo sapiens ]
• Official Symbol: SNCA
• Synonyms: SNCA; synuclein, alpha (non A4 component of amyloid precursor); PARK1, PARK4, Parkinson disease (autosomal dominant, Lewy body) 4; alpha-synuclein; alpha synuclein; NACP; PD1; synuclein alpha-140; non A-beta component of AD amyloid; PARK1; PARK4; MGC110988
• Gene ID: 6622
• mRNA Refseq: NM_000345
• Protein Refseq: NP_000336
• MIM: 163890
• UniProt ID: P37840
• Chromosome Location: 4q21.3-q22
• Pathway: Alpha-synuclein signaling, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amyloids, organism-specific biosystem; Disease, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Parkinsons disease, organism-specific biosystem
• Function: Hsp70 protein binding; alpha-tubulin binding; arachidonic acid binding; calcium ion binding; copper ion binding; cysteine-type endopeptidase inhibitor activity involved in apoptotic process; dynein binding; NOT fatty acid binding; ferrous iron binding; histone binding; identical protein binding; kinesin binding; magnesium ion binding; oxidoreductase activity; NOT phospholipase D inhibitor activity; phosphoprotein binding; protein binding; tau protein binding; zinc ion binding