Recombinant Human SMOX protein, His & T7-tagged

• Cat.No.: SMOX-8111H
• Product Overview: Recombinant Human SMOX aa. (Met1~Gln165 (Accession # Q9NWM0)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Polyamines are ubiquitous polycationic alkylamines which include spermine, spermidine, putrescine, and agmatine. These molecules participate in a broad range of cellular functions which include cell cycle modulation, scavenging reactive oxygen species, and the control of gene expression. These molecules also play important roles in neurotransmission through their regulation of cell-surface receptor activity, involvement in intracellular signalling pathways, and their putative roles as neurotransmitters. This gene encodes an FAD-containing enzyme that catalyzes the oxidation of spermine to spermadine and secondarily produces hydrogen peroxide. Multiple transcript variants encoding different isoenzymes have been identified for this gene, some of which have failed to demonstrate significant oxidase activity on natural polyamine substrates. The characterized isoenzymes have distinctive biochemical characteristics and substrate specificities, suggesting the existence of additional levels of complexity in polyamine catabolism.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 21.6kDa
• Protein length: Met1~Gln165
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 5.8.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: SMOX spermine oxidase [ Homo sapiens (human) ]
• Official Symbol: SMOX
• Synonyms: PAO; SMO; PAO1; PAOH; PAO-1; PAOH1; C20orf16; flavin containing amine oxidase; flavin-containing spermine oxidase; polyamine oxidase 1; putative cyclin G1 interacting protein; spermine oxidase
• Gene ID: 54498
• mRNA Refseq: NM_001270691.1
• Protein Refseq: NP_001257620.1
• UniProt ID: Q9NWM0