Recombinant Human SMG8 protein, His-tagged

• Cat.No.: SMG8-3147H
• Product Overview: Recombinant Human SMG8 protein(521-745 aa), fused to His tag, was expressed in E. coli.

Specification

• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 521-745 aa
• AA Sequence: MTVHKNQLAQALRVYSQHARGPAFHKYAMQLHEDCYKFWSNGHQLCEERSLTDQHCVHKFHSLPKSGEKPEADRNPPVLYHNSRARSTGACNCGRKQAPRDDPFDIKAANYDFYQLLEEKCCGKLDHINFPVFEPSTPDPAPAKNESSPAPPDSDADKLKEKEPQTQGESTSLSLALSLGQSTDSLGTYPADPQAGGDNPEVHGQVEVKTEKRPNFVDRQASTVE
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: SMG8 smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans) [ Homo sapiens ]
• Official Symbol: SMG8
• Synonyms: SMG8; smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans); C17orf71, chromosome 17 open reading frame 71; protein SMG8; FLJ10587; FLJ23205; protein smg-8 homolog; amplified in breast cancer gene 2 protein; C17orf71
• Gene ID: 55181
• mRNA Refseq: NM_018149
• Protein Refseq: NP_060619
• MIM: 613175
• UniProt ID: Q8ND04