Recombinant Mouse Smg8 Protein, Myc/DDK-tagged

• Cat.No.: Smg8-5967M
• Product Overview: Purified recombinant protein of mouse full-length smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg8), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG9 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required to mediate the recruitment of SMG1 to the ribosome:SURF complex and to suppress SMG1 kinase activity until the ribosome:SURF complex locates the exon junction complex (EJC). Acts as a regulator of kinase activity.
• Molecular Mass: 109.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Smg8 smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans) [ Mus musculus (house mouse) ]
• Official Symbol: Smg8
• Synonyms: Smg8; smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans); 1200011M11Rik; protein SMG8; CG6729 gene product; protein smg-8 homolog
• Gene ID: 74133
• mRNA Refseq: NM_024262
• Protein Refseq: NP_077224
• UniProt ID: Q8VE18