Recombinant Human Small Nuclear Ribonucleoprotein Polypeptide A', His-tagged

• Cat.No.: SNRPA1-4830H
• Product Overview: Recombinant human SNRPA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Cat. No.: SNRPA1-4830H
• Description: SNRPA1, also known as U2 small nuclear ribonucleoprotein A, is a component of the U2 snRNP that forms a complex with U2 snRNP B (U2B). Together, U2 snRNP A and U2 snRNP B form a complex that binds to the U2 snRNA hairpin IV. The configuration of this U2 snRNP A/U2 snRNP B dimer and the subtle variations of a few key residues regulate the snRNP-RNA-binding specificity. U2 snRNP A is a 255 amino acid protein, and two isoforms exist as a result of alternative splicing events.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing30% glycerol, 0.2M NaCl, 2mM DTT, 0.1mM PMSF.
• Molecular Weight: 30.5 kDa (275aa), confirmed by MALDI-TOF.
• Purity: >80% by SDS-PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLLVNNNRICRIG EGLDQALPCL TELILTNNSL VELGDLDPLA SLKSLTYLSI LRNPVTNKKH YRLYVIYKVP QVRVLDFQKV KLKERQEAEKMFKGKRGAQL AKDIARRSKT FNPGAGLPTD KKKGGPSPGD VEAIKNAIAN ASTLAEVERL KGLLQSGQIP GRERRSGPTD DGEEEMEEDTVTNGS
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Formation and Maturation of mRNA Transcript; Gene Expression; Processing of Capped Intron-Containing Pre-mRNA; Spliceosome; mRNA Processing; mRNA Splicing; mRNA Splicing - Major Pathway; mRNA processing

Gene Information

• Gene Name: SNRPA1 small nuclear ribonucleoprotein polypeptide A" [ Homo sapiens ]
• Official Symbol: SNRPA1
• Synonyms: SNRPA1; small nuclear ribonucleoprotein polypeptide A"; Lea1; U2 small nuclear ribonucleoprotein A"; U2 snRNP A"; OTTHUMP00000194836; U2 small nuclear ribonucleoprotein polypeptide A"
• Gene ID: 6627
• mRNA Refseq: NM_003090
• Protein Refseq: NP_003081
• MIM: 603521
• UniProt ID: P09661
• Chromosome Location: 15q26.3
• Function: RNA binding; protein binding