Recombinant Human SMAD Family Member 4, GST-tagged

• Cat.No.: SMAD4-1095H
• Product Overview: Recombinant full-length human SMAD4 was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: SMAD4 is a member of the SMAD family and mediates signaling by the transforming growth factor-beta superfamily and related ligands. TGFβ stimulation leads to phosphorylation and activation of SMAD1, SMAD2 and SMAD3, which form complexes with SMAD4 that accumulate in the nucleus and regulate transcription of target genes. SMAD signaling is negatively regulated by inhibitory SMADs and ubiquitin-mediated processes and proteasomal degradation of SMADs depend on the direct interaction of specific E3 ligases with SMADs.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 95 kDa
• Expression System: E. coli
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1mM PMSF, 25 % glycerol.
• Purity: > 80 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: ALK1 signaling events; ALK2 signaling events; Adherens junction; Cell cycle; Colorectal cancer; HTLV-I infection; Pancreatic cancer; Pathways in cancer; Regulation of cytoplasmic and nuclear SMAD2/3 signaling; Regulation of nuclear SMAD2/3 signaling; Signaling by TGF beta; TGF Beta Signaling Pathway; Wnt signaling pathway; BMP receptor signaling; Heart Development

Gene Information

• Gene Name: SMAD4 SMAD family member 4 [ Homo sapiens ]
• Official Symbol: SMAD4
• Synonyms: SMAD4; SMAD family member 4; JIP; DPC4; MADH4; mothers against decapentaplegic homolog 4; MAD homolog 4; OTTHUMP00000163548; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4; MAD, mothers against decapentaplegic homolog 4 (Drosophila); SMAD, mothers against DPP homolog 4 (Drosophila); Mothers against decapentaplegic homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; Smad4
• Gene ID: 4089
• mRNA Refseq: NM_005359
• Protein Refseq: NP_005350
• MIM: 600993
• UniProt ID: Q13485
• Chromosome Location: 18q21.1
• Function: R-SMAD binding; protein binding; transforming growth factor beta receptor; contributes to DNA binding; sequence-specific DNA binding transcription factor activity; core promoter proximal region sequence-specific DNA binding; transcription regulatory region DNA binding; DNA binding; I-SMAD binding; SMAD binding; chromatin binding; collagen binding; identical protein binding; contributes to protein binding transcription factor activity; protein homodimerization activity