Recombinant Human SLC7A1 293 Cell Lysate

• Cat.No.: SLC7A1-1700HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 (SLC7A1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SLC7A1 solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 [ Homo sapiens ]
• Official Symbol: SLC7A1
• Synonyms: SLC7A1; solute carrier family 7 (cationic amino acid transporter, y+ system), member 1; ATRC1, ERR; high affinity cationic amino acid transporter 1; amino acid transporter; cationic 1; CAT 1; ecotropic retroviral receptor; HCAT1; REC1L; CAT1; solute carrier family 7 member 1; amino acid transporter, cationic 1; ecotropic retrovirus receptor homolog; system Y+ basic amino acid transporter; ecotropic retroviral leukemia receptor homolog; ERR; ATRC1; CAT-1
• Gene ID: 6541
• mRNA Refseq: NM_003045
• Protein Refseq: NP_003036
• MIM: 104615
• UniProt ID: P30825
• Chromosome Location: 13q12-q14
• Pathway: Amino acid transport across the plasma membrane, organism-specific biosystem; SLC-mediated transmembrane transport, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem; Transport of inorganic cations/anions and amino acids/oligopeptides, organism-specific biosystem
• Function: amino acid transmembrane transporter activity; arginine transmembrane transporter activity; receptor activity