Recombinant Human SIRPG protein, His-Avi-tagged

• Cat.No.: SIRPG-918H
• Product Overview: Recombinant Human SIRPG protein(Q9P1W8)(Glu29-Pro360), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His-Avi
• Protein length: Glu29-Pro360
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human SIRP Gamma, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 2.46μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: SIRPG signal-regulatory protein gamma [ Homo sapiens ]
• Official Symbol: SIRPG
• Synonyms: SIRPG; signal-regulatory protein gamma; signal regulatory protein beta 2 , SIRPB2; bA77C3.1; CD172g; SIRP B2; SIRPgamma; SIRP-gamma; SIRP beta 2; SIRP-beta-2; CD172g antigen; signal-regulatory protein beta 2; signal-regulatory protein beta-2; CD172 antigen-like family member B; SIRPB2; SIRP-B2; FLJ42230
• Gene ID: 55423
• mRNA Refseq: NM_001039508
• Protein Refseq: NP_001034597
• MIM: 605466
• UniProt ID: Q9P1W8