Recombinant Human SIRPA protein, hFc-Avi-tagged, Biotinylated

• Cat.No.: SIRPA-324HB
• Product Overview: Biotinylated Recombinant Human SIRPA protein(P78324)(Glu31-Arg370), fused with C-terminal hFc and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc-Avi
• Protein length: Glu31-Arg370
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SIRP alpha, hFc Tag with the EC50 of 0.22ug/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 66.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: SIRPA signal-regulatory protein alpha [ Homo sapiens ]
• Official Symbol: SIRPA
• Synonyms: SIRPA; signal-regulatory protein alpha; protein tyrosine phosphatase, non receptor type substrate 1 , PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; MYD-1; CD172A; PTPNS1
• Gene ID: 140885
• mRNA Refseq: NM_001040022
• Protein Refseq: NP_001035111
• MIM: 602461
• UniProt ID: P78324