Active Recombinant Human SIRPA Protein, His & Avi-tagged, Biotinylated

• Cat.No.: SIRPA-347H
• Product Overview: Recombinant Human SIRPA Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111). Predicted N-terminus: Glu 31. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 39.9 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Glu 31 - Arg 370
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD47, Fc Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human SIRPA Protein, His & Avi-tagged, Biotinylated with a linear range of 0.078-0.625 μg/mL (QC tested).
• Molecular Mass: 39.9 kDa
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >95% as determined by SEC-HPLC.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: SIRPA signal-regulatory protein alpha [ Homo sapiens ]
• Official Symbol: SIRPA
• Synonyms: SIRPA; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; MYD-1; CD172A; PTPNS1
• Gene ID: 140885
• mRNA Refseq: NM_001040022
• Protein Refseq: NP_001035111
• MIM: 602461
• UniProt ID: P78324