Active Recombinant Human SIRPA protein, His-tagged

• Cat.No.: SIRPA-1276H
• Product Overview: Recombinant Human SIRP-alpha/CD172a Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Glu31-Arg370) of human SIRP alpha/CD172a (Accession #NP_542970) fused with a 6×His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Glu31-Arg370
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human SIRPA at 5 μg/mL (100 μL/well) can bind Recombinant Human CD47 with a linear range of 0.1-0.4 μg/mL.
• Molecular Mass: 45-57 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 97% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: SIRPA signal-regulatory protein alpha [ Homo sapiens ]
• Official Symbol: SIRPA
• Synonyms: SIRPA; signal-regulatory protein alpha; protein tyrosine phosphatase, non receptor type substrate 1 , PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; MYD-1; CD172A; PTPNS1
• Gene ID: 140885
• mRNA Refseq: NM_001040022
• Protein Refseq: NP_001035111
• MIM: 602461
• UniProt ID: P78324