Recombinant Human SIGLEC15 protein, hFc-tagged

• Cat.No.: SIGLEC15-6744H
• Product Overview: Recombinant Human SIGLEC15 protein(Q6ZMC9-1)(Lys24-Gly260), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• ProteinLength: Lys24-Gly260
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in 20mM NaAC (pH 5.0). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Siglec-15, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 22.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAC (pH 5.0).