Recombinant Human SIAH1 293 Cell Lysate

• Cat.No.: SIAH1-1851HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SIAH1 siah E3 ubiquitin protein ligase 1 [ Homo sapiens ]
• Official Symbol: SIAH1
• Synonyms: SIAH1; siah E3 ubiquitin protein ligase 1; seven in absentia homolog 1 (Drosophila); E3 ubiquitin-protein ligase SIAH1; hSIAH1; siah-1a; seven in absentia homolog 1; SIAH1A; FLJ08065
• Gene ID: 6477
• mRNA Refseq: NM_001006610
• Protein Refseq: NP_001006611
• MIM: 602212
• UniProt ID: Q8IUQ4
• Chromosome Location: 16q12
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Netrin-1 signaling, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem; Ubiquitin mediated proteolysis, conserved biosystem; Wnt signaling pathway, organism-specific biosystem; Wnt signaling pathway, conserved biosystem
• Function: ligase activity; metal ion binding; protein C-terminus binding; protein binding; ubiquitin-protein ligase activity; ubiquitin-protein ligase activity; zinc ion binding; zinc ion binding