Recombinant Human SHC1, His-tagged

• Cat.No.: SHC1-30065TH
• Product Overview: Recombinant fragment from Human SHC1 (aa 1-474) with N terminal His-tag; 494aa (53.8 kDa), inclusive of tag.

Specification

• Description: This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
• Protein length: 474 amino acids
• Conjugation: HIS
• Molecular Weight: 53.800kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Widely expressed. Expressed in neural stem cells but absent in mature neurons.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 200mM Sodium chloride, 20mM Tris HCl, 2mM DTT, 0.1mM PMSF, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHFNTRTQVTREAISLVCEAVPGAKGATRRRKPCSRPLSSILGRSNLKFAGMPITLTVSTSSLNLMAADCKQIIANHHMQSISFASGGDPDTMNKLSGGGGRRTRVEGGQLGGEEWTRHGSFVNKPTRGWLHPNDKVMGPGVSYLVRYMGCVEVLQSMRALDAEYVAYVAKDPVNQRACHILECPEGLAQDVISTIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGSAWDEEEEEPPDHQYYNDFPGKEPPLGGVVDMRLREGAAPGAARPTAPNAQTPSHLGATLPVGQPVGGDPEVRKQMPPPPPCPAGRELFDDPSYVNVQNLDKARQAVGGAGPPNPAINGSAPRDLFDMKPFEDALRVPPPPQSVSMAEQLRGEPWFHGKLSRREAEALLQLNGDFLVRESTTTPGQYVLTGLQSGQPKHLLLVDPEGVVRTKDHRFESVSHLISYHMDNHLPIISAGSELCLQQPVERKL
• Sequence Similarities: Contains 1 PID domain.Contains 1 SH2 domain.

Gene Information

• Gene Name: SHC1 SHC (Src homology 2 domain containing) transforming protein 1 [ Homo sapiens ]
• Official Symbol: SHC1
• Synonyms: SHC1; SHC (Src homology 2 domain containing) transforming protein 1; SHC, SHC (Src homology 2 domain containing) transforming protein 1; SHC-transforming protein 1; p66
• Gene ID: 6464
• mRNA Refseq: NM_001130040
• Protein Refseq: NP_001123512
• MIM: 600560
• Uniprot ID: P29353
• Chromosome Location: 1q21
• Pathway: Activation of Chaperones by IRE1alpha, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem
• Function: ephrin receptor binding; epidermal growth factor receptor binding; epidermal growth factor receptor binding; insulin receptor binding; insulin-like growth factor receptor binding