Recombinant Human SHC1 293 Cell Lysate

• Cat.No.: SHC1-1862HCL

Specification

• Description: Antigen standard for SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: SHC1 SHC (Src homology 2 domain containing) transforming protein 1 [ Homo sapiens ]
• Official Symbol: SHC1
• Synonyms: SHC1; SHC (Src homology 2 domain containing) transforming protein 1; SHC, SHC (Src homology 2 domain containing) transforming protein 1; SHC-transforming protein 1; p66; SH2 domain protein C1; SHC-transforming protein 3; SHC-transforming protein A; SHC (Src homology 2 domain-containing) transforming protein 1; SHC; SHCA; FLJ26504
• Gene ID: 6464
• mRNA Refseq: NM_001130040
• Protein Refseq: NP_001123512
• MIM: 600560
• UniProt ID: P29353
• Chromosome Location: 1q21
• Pathway: Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Antigen Activates B Cell Receptor Leading to Generation of Second Messengers, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem
• Function: ephrin receptor binding; ephrin receptor binding; epidermal growth factor receptor binding; epidermal growth factor receptor binding; insulin receptor binding; insulin-like growth factor receptor binding; neurotrophin TRKA receptor binding; phospholipid binding; phosphoprotein binding; phosphotyrosine binding; protein binding; protein complex binding; protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase adaptor activity