Recombinant Human SHARPIN Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: SHARPIN-1033H
• Product Overview: SHARPIN MS Standard C13 and N15-labeled recombinant protein (NP_112236) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 39.8 kDa
• AA Sequence: MAPPAGGAAAAASDLGSAAVLLAVH AAVRPLGAGPDAEAQLRRLQLSADP ERPGRFRLELLGAGPGAVNLEWPLE SVSYTIRGPTQHELQPPPGGPGTLS LHFLNPQEAQRWAVLVRGATVEGQN GSKSNSPPALGPEACPVSLPSPPEA STLKGPPPEADLPRSPGNLTEREEL AGSLARAIAGGDEKGAAQVAAVLAQ HRVALSVQLQEACFPPGPIRLQVTL EDAASAASAASSAHVALQVHPHCTV AALQEQVFSELGFPPAVQRWVIGRC LCVPERSLASYGVRQDGDPAFLYLL SAPREAPATGPSPQHPQKMDGELGR LFPPSLGLPPGPQPAASSLPSPLQP SWSCPSCTFINAPDRPGCEMCSTQR PCTWDPLAAASTTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SHARPIN SHANK-associated RH domain interactor [ Homo sapiens (human) ]
• Official Symbol: SHARPIN
• Synonyms: SHARPIN; SHANK-associated RH domain interactor; sharpin; DKFZP434N1923; SIPL1; hSIPL1; shank-interacting protein-like 1; shank-associated RH domain-interacting protein; DKFZp434N1923
• Gene ID: 81858
• mRNA Refseq: NM_030974
• Protein Refseq: NP_112236
• MIM: 611885
• UniProt ID: Q9H0F6