Recombinant Human SHARPIN protein, His-tagged

• Cat.No.: SHARPIN-3420H
• Product Overview: Recombinant Human SHARPIN protein(1-326 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 1-326 aa
• AA Sequence: MAPPAGGAAAAASDLGSAAVLLAVH AAVRPLGAGPDAEAQLRRLQLSADP ERPGRFRLELLGAGPGAVNLEWPLE SVSYTIRGPTQHELQPPPGGPGTLS LHFLNPQEAQRWAVLVRGATVEGQN GSKSNSPPALGPEACPVSLPSPPEA STLKGPPPEADLPRSPGNLTEREEL AGSLARAIAGGDEKGAAQVAAVLAQ HRVALSVQLQEACFPPGPIRLQVTL EDAASAASAASSAHVALQVHPHCTV AALQEQVFSELGFPPAVQRWVIGRC LCVPERSLASYGVRQDGDPAFLYLL SAPREAPAQLVLSFLHLHQCPRPPW L
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: SHARPIN SHANK-associated RH domain interactor [ Homo sapiens ]
• Official Symbol: SHARPIN
• Synonyms: SHARPIN; SHANK-associated RH domain interactor; sharpin; DKFZP434N1923; SIPL1; hSIPL1; shank-interacting protein-like 1; shank-associated RH domain-interacting protein; DKFZp434N1923
• Gene ID: 81858
• mRNA Refseq: NM_030974
• Protein Refseq: NP_112236
• MIM: 611885
• UniProt ID: Q9H0F6