Recombinant Human SGK3 protein(Met1-Leu496), His&GST-tagged

• Cat.No.: SGK3-7328H
• Product Overview: Recombinant Human SGK3 (Q96BR1-1) (Met 1-Leu 496) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-496 a.a.
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 0.5mM PMSF, 10% gly, 1mM GSH, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human SGK3/GST chimera consists of 733 amino acids and has a calculated molecular mass of 85 kDa. It migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SGK3 serum/glucocorticoid regulated kinase family, member 3 [ Homo sapiens ]
• Official Symbol: SGK3
• Synonyms: SGK3; serum/glucocorticoid regulated kinase family, member 3; serum/glucocorticoid regulated kinase like , SGK2, SGKL; serine/threonine-protein kinase Sgk3; cytokine-independent survival kinase; CISK; SGK2; SGKL; DKFZp781N0293
• Gene ID: 23678
• mRNA Refseq: NM_001033578
• Protein Refseq: NP_001028750
• MIM: 607591
• UniProt ID: Q96BR1